GRIN2B antibody (pTyr1252)

Catalog No. ABIN6658236

Supplier Product No.: 612-401-d92

This Rabbit Polyclonal antibody specifically detects GRIN2B in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for GRIN2B antibody (pTyr1252) (ABIN6658236)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GRIN2B Antibody

Binding Specificity: pTyr1252

Supplier Product No.: 612-401-d92

Supplier: Rockland

Purpose: NMDA R2B phospho Y1252 Antibody

Cross-Reactivity (Details): NMDA R2B pY1252 antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Purification: Anti-NMDA R2B pY1252 antibody is directed against rat NMDA R2B protein.

Immunogen: Anti-NMDA R2B pY1252 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:400

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NMDA R2B pY1252 Antibody is tested for use in Western Blotting and IHC. Expect a band of approximately 180 kDa in size corresponding to NMDAR NR2B subunit protein phosphorylated at Tyr1252 in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: NMDA R2B

Background:

Synonyms: Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b

Background: NMDA R2B pY1252 Antibody detects NMDA R2B protein. The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Phosphorylation of Tyr1252 is thought to potentiate NMDA receptor-dependent influx of calcium. Anti-NMDA R2B pY1252 Antibody is ideal for investigators involved in Neuroscience, Signal Transduction, and Cell Signaling Research.

Gene Name: GRIN2B

Gene ID: 24410

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity