PLK1 antibody (pSer482, pSer486, pSer490, pSer392482)

Catalog No. ABIN6658298

Supplier Product No.: 600-401-e12

This anti-PLK1 antibody is a Rabbit Polyclonal antibody detecting PLK1 in WB. Suitable for Xenopus laevis.

Quick Overview for PLK1 antibody (pSer482, pSer486, pSer490, pSer392482) (ABIN6658298)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLK1 Antibody

Binding Specificity: pSer482, pSer486, pSer490, pSer392482

Supplier Product No.: 600-401-e12

Supplier: Rockland

Purpose: Polo-Like Kinase phospho S482/phospho S486/phospho S490 Antibody

Cross-Reactivity (Details): Anti-Polo-Like Kinase pS482/pS486/pS490 antibody is directed against phosphorylated Polo-Like Kinase protein.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Polo-like Kinase pS482/pS486/pS490 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392482/486/490.

Isotype: IgG

Application Details

Application Notes:

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-Polo-Like Kinase pS482/pS486/pS490 Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 120 kDa in size corresponding to PLKK protein phosphorylated at Ser482/486/490 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: Polo-Like Kinase

Background:

Synonyms: Serine/threonine-protein kinase PLK1, Plx1

Background: Polo-Like Kinase pS482/pS486/pS490 detects Polo-Like Kinase proteins. Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK. Anti-Polo-Like Kinase pS482/pS486/pS490 Antibody is ideal for investigators involved in Cell Cycle and Apoptosis research.

Gene Name: PLK1

Gene ID: 380481

Pathways: Cell Division Cycle, M Phase