Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: 1:10,000
IF Microscopy Dilution: 1:1,000 - 1:5,000