Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN6699200
Antigen
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Human
827
313
306
132
117
77
67
64
52
44
42
40
19
16
11
10
8
5
5
3
2
2
2
2
1
1
1
1
1
1
Host
Chicken
414
389
28
12
11
8
7
3
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
35
24
22
17
14
11
11
11
11
11
11
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
655
331
228
210
192
183
92
63
53
51
41
22
15
14
13
9
8
3
3
3
1
1
1
1
1
1
1
Options
Immunogen

Immunogen: Anti-Vimentin Monoclonal Antibody was produced in chicken by repeated immunizations with recombinant human vimentin extensively purified from E. coli.

Immunogen Type: Recombinant Protein

Isotype IgG
Cross-Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Cross reactivity with Vimentin from other species has not been determined.
Purification Anti-Vimentin antibody is directed against human Vimentin proteins. The antibody was purified from chicken eggs as an IgY fraction. Reactivity is expected from human, mouse and rat sources.
Plasmids, Primers & others Plasmids, Primers & others Vimentin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background

Synonyms: VIM

Background: Vimentin antibody recognizes Vimentin which is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin antibody is ideal for investigators involved in Neuroscience, Cell Migration and Cancer Research.

Gene Name: VIM

Gene ID 7431
UniProt P08670
Pathways Caspase Cascade in Apoptosis
Application Notes

Application Note: Anti-Vimentin antibody is suitable for use in ELISA and Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the vimentin proteins in Western blots in the appropriate cell lysate or extract.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:500

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Supplier Images
Image no. 1 for anti-Vimentin (VIM) antibody (ABIN6699200) IF - Vimentin Immunofluorescence Microscopy of Chicken anti-Vimentin antibody. Tissue...
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