TIGAR antibody (AA 161-270) (HRP)

Catalog No. ABIN671295

The HRP-conjugated Rabbit Polyclonal anti-TIGAR antibody (ABIN671295) specifically detects TIGAR in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for TIGAR antibody (AA 161-270) (HRP) (ABIN671295)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TIGAR Antibody (HRP)

Binding Specificity: AA 161-270

Cross-Reactivity: Mouse

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TIGAR

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR

Background:

Synonyms: FR2BP, TIGAR, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, C12orf5

Background: Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6-bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53.

Gene ID: 57103

UniProt: Q9NQ88

Pathways: Warburg Effect