FGF2 antibody (AA 143-288)

Catalog No. ABIN6719317

This Rabbit Polyclonal antibody specifically detects FGF2 in WB, ELISA, IHC, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for FGF2 antibody (AA 143-288) (ABIN6719317)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-FGF2 Antibody

Binding Specificity: AA 143-288

Purpose: Anti-FGF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FGF2 Antibody Picoband® (ABIN6719317). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human FGF2 recombinant protein (Position: P143-S288).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry, 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
ELISA (Cap), 1-5 μg/mL
1. Doniach, T. : Basic FGF as an inducer of anteroposterior neural pattern. Cell 83: 1067-1070, 1995. 2. Eckenstein, F. P. : Fibroblast growth factors in the nervous system. J. Neurobiol. 25: 1467-1480, 1994.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF2

Background:

Synonyms: Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2, FGF2, FGFB

Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.

Background: FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96 % and 97 % amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Molecular Weight: 17-21 kDa

Gene ID: 2247

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins