DOK7 antibody
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- Target See all DOK7 Antibodies
- DOK7 (Docking Protein 7 (DOK7))
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DOK7 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Purpose
- Rabbit IgG polyclonal antibody for DOK7 detection. Tested with WB, IHC-P, ICC/IF in Human,Mouse,Rat.
- Sequence
- STVEERVAQE ALETLQLEKR LSLLSHAGR
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
- Rabbit IgG polyclonal antibody for DOK7 detection. Tested with WB, IHC-P, ICC/IF in Human,Mouse,Rat.
- Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence of human DOK7(STVEERVAQEALETLQLEKRLSLLSHAGR).
- Isotype
- IgG
- Top Product
- Discover our top product DOK7 Primary Antibody
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- Application Notes
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Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Immunocytochemistry/Immunofluorescence|2 μg/mL
- Comment
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- DOK7 (Docking Protein 7 (DOK7))
- Alternative Name
- DOK7 (DOK7 Products)
- Synonyms
- si:dkey-180b4.3 antibody, si:ch211-164d19.2 antibody, C4orf25 antibody, CMS1B antibody, A930013K19Rik antibody, AW049091 antibody, Dok-7 antibody, EF-12 antibody, Oit5 antibody, RGD1566416 antibody, dok-7 antibody, docking protein 7 antibody, DOK7 antibody, dok7 antibody, Dok7 antibody
- Background
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Synonyms: Protein Dok-7, Downstream of tyrosine kinase 7, DOK7, C4orf25,
Background: Dok-7 is a non-catalytic cytoplasmic adaptor protein that is expressed specifically in muscle and is essential for the formation of neuromuscular synapses. Further, Dok-7 contains pleckstrin homology (PH) and phosphotyrosine-binding (PTB) domains that are critical for Dok-7 function. It is mapped to 4p16.3. The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants.
- Gene ID
- 285489
- Pathways
- Skeletal Muscle Fiber Development
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