BAX antibody (N-Term) (DyLight 550)

Catalog No. ABIN7354485

The Rabbit Polyclonal anti-BAX antibody has been validated for FACS. It is suitable to detect BAX in samples from Human.

Quick Overview for BAX antibody (N-Term) (DyLight 550) (ABIN7354485)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-BAX Antibody (DyLight 550)

Binding Specificity: AA 17-48, N-Term

Purpose: Anti-Human Bax DyLight® 550 conjugated Antibody

Sequence: EQIMKTGALL LQGFIQDRAG RMGGEAPELA LD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human Bax DyLight® 550 conjugated Antibody (ABIN5518740)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Bax, different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Apte, S. S., Mattei, M.-G., Olsen, B. R. : Mapping of human BAX gene to chromosome 19q13.3-q13.4 and isolation of a novel alternatively spliced transcript, BAX-delta. Genomics 26: 592-594, 1995. 2. Guo, B., Zhai, D., Cabezas, E., Welsh, K., Nouraini, S., Satterthwait, A. C., Reed, J. C. : Humanin peptide suppresses apoptosis by interfering with Bax activation. Nature 423: 456-461, 2003. 3. Oltvai, Z. N., Milliman, C. L., Korsmeyer, S. J. : Bcl-2 heterodimers in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619, 1993. 4. Takeuchi, O., Fisher, J., Suh, H., Harada, H., Malynn, B. A., Korsmeyer, S. J. : Essential role of BAX,BAK in B cell homeostasis and prevention of autoimmune disease. Proc. Nat. Acad. Sci. 102: 11272-11277, 2005.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX

Background:

Synonyms: Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BAX, BCL2L4

Tissue Specificity: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Background: Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Molecular Weight: 39 kDa

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response