JAK3 antibody (AA 406-661)

Catalog No. ABIN6719493

This anti-JAK3 antibody is a Rabbit Polyclonal antibody detecting JAK3 in WB and ELISA. Suitable for Human.

Quick Overview for JAK3 antibody (AA 406-661) (ABIN6719493)

Target: JAK3 (Janus Kinase 3 (JAK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-JAK3 Antibody

Binding Specificity: AA 406-661

Purpose: Anti-JAK3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-JAK3 Antibody Picoband® (ABIN6719493). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human JAK3 recombinant protein (Position: Q406-D661).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Riedy MC, Dutra AS, Blake TB, Modi W, Lal BK, Davis J, Bosse A, O'Shea JJ, Johnston JA (October 1996). "Genomic sequence, organization, and chromosomal localization of human JAK3". Genomics. 37 (1): 57-61. 2. Hoffman SM, Lai KS, Tomfohrde J, Bowcock A, Gordon LA, Mohrenweiser HW (July 1997). "JAK3 maps to human chromosome 19p12 within a cluster of proto-oncogenes and transcription factors". Genomics. 43 (1): 109-11.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for JAK3

Target: JAK3 (Janus Kinase 3 (JAK3))

Alternative Name: JAK3

Background:

Synonyms: Tyrosine-protein kinase JAK3, Janus kinase 3, JAK-3, Leukocyte janus kinase, L-JAK, JAK3

Tissue Specificity: In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

Background: Tyrosine-protein kinase JAK3 is an enzyme that in humans is encoded by the JAK3 gene. It is mapped to 19p13.11. The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).

Molecular Weight: 125 kDa

Gene ID: 3718

UniProt: P52333

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response