GFP antibody (DyLight 800)

Catalog No. ABIN6720709

Supplier Product No.: 600-145-215

This Goat Polyclonal antibody specifically detects GFP in WB, ELISA, FM, DB and FLISA. It exhibits reactivity toward Aequorea victoria and has been mentioned in 4+ publications.

Quick Overview for GFP antibody (DyLight 800) (ABIN6720709)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to DyLight 800

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Product Details anti-GFP Antibody (DyLight 800)

Supplier Product No.: 600-145-215

Supplier: Rockland

Purpose: GFP Antibody Dylight™ 800 Conjugated

Specificity: Wt, rGFP, eGFP

Cross-Reactivity (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Purification: GFP Dylight™ 800 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Isotype: IgG

Labeling Ratio: 2.3

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:20,000 - 1:40,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000-1:25,000

Other: User Optimized

Comment:

Suggested Applications: WB
Anti-GFP Dylight™ 800 Conjugated Antibody has been tested by dot blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GFP antibody (DyLight 800) (ABIN6720709)

Frolova, Palchevska, Dominguez, Frolov: "Alphavirus-induced transcriptional and translational shutoffs play major roles in blocking the formation of stress granules." in: Journal of virology, Vol. 97, Issue 11, pp. e0097923, (2023) (PubMed).

Dominguez, Palchevska, Frolova, Frolov: "Alphavirus-based replicons demonstrate different interactions with host cells and can be optimized to increase protein expression." in: Journal of virology, Vol. 97, Issue 11, pp. e0122523, (2023) (PubMed).

Frolov, Agback, Palchevska, Dominguez, Lomzov, Agback, Frolova: "All Domains of SARS-CoV-2 nsp1 Determine Translational Shutoff and Cytotoxicity of the Protein." in: Journal of virology, Vol. 97, Issue 3, pp. e0186522, (2023) (PubMed).

Czarna, Wang, Zelencova, Liu, Deng, Choi, Zhang, Zhou, Chang, Kildalsen, Seternes, Gray, Engh, Rothweiler: "Novel Scaffolds for Dual Specificity Tyrosine-Phosphorylation-Regulated Kinase (DYRK1A) Inhibitors." in: Journal of medicinal chemistry, Vol. 61, Issue 17, pp. 7560-7572, (2019) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Synonyms: goat anti-GFP Antibody DyLight™ 800 Conjugation, DyLight™ 800 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: GFP Dylight™ 800 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms such as the LICOR Odyssey Imaging system.

UniProt: P42212