HIF1A antibody (AA 341-450)

Catalog No. ABIN672546

This anti-HIF1A antibody is a Rabbit Polyclonal antibody detecting HIF1A in WB, ELISA, IP, FACS, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Human, Mouse, Rat and Rabbit. This Primary Antibody has been cited in 10+ publications.

Quick Overview for HIF1A antibody (AA 341-450) (ABIN672546)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HIF1A Antibody

Binding Specificity: AA 341-450

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HIF1A antibody (ABIN672546)

Talwar, Bauerfeld, Bouhamdan, Farshi, Liu, Samavati: "MKP-1 negatively regulates LPS-mediated IL-1β production through p38 activation and HIF-1α expression." in: Cellular signalling, Vol. 34, pp. 1-10, (2017) (PubMed).

Talwar, Bauerfeld, Liu, Samavati: "The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages." in: Data in brief, Vol. 14, pp. 56-61, (2017) (PubMed).

Madka, Mohammed, Li, Zhang, Biddick, Patlolla, Lightfoot, Towner, Wu, Steele, Kopelovich, Rao: "Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo." in: Cancer prevention research (Philadelphia, Pa.), Vol. 9, Issue 1, pp. 53-62, (2016) (PubMed).

Yang, Lu, Guo: "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." in: Molecular medicine reports, Vol. 14, Issue 5, pp. 4075-4082, (2016) (PubMed).

Zhang, Ren, Tang, Wang, Liu, Zhang, Li, Liu, Zhao, He: "Vascular normalization induced by sinomenine hydrochloride results in suppressed mammary tumor growth and metastasis." in: Scientific reports, Vol. 5, pp. 8888, (2015) (PubMed).

Woolf, Curley, Liu, Turner, Charlton, Preul, Scheck: "The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130357, (2015) (PubMed).

Yang, Li, Yan, Zhang, Sun, Qu, Wang, Duan: "Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer." in: Oncology reports, Vol. 34, Issue 5, pp. 2385-94, (2015) (PubMed).

Fan, Xu, Li, Tie, Pan, Li: "Opposite angiogenic outcome of curcumin against ischemia and Lewis lung cancer models: in silico, in vitro and in vivo studies." in: Biochimica et biophysica acta, Vol. 1842, Issue 9, pp. 1742-54, (2014) (PubMed).

Guo, Feng, Yao, Sun, Zhu: "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." in: BMC nephrology, Vol. 15, pp. 110, (2014) (PubMed).

Shou, Lin, Liang, Li, Chen: "Expression and prognosis of FOXO3a and HIF-1? in nasopharyngeal carcinoma." in: Journal of cancer research and clinical oncology, Vol. 138, Issue 4, pp. 585-93, (2012) (PubMed).

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF-1 alpha

Background:

Synonyms: HIF1, MOP1, PASD8, HIF-1A, bHLHe78, HIF-1alpha, HIF1-ALPHA, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A

Background: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 4 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP3. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Gene ID: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect