p21 antibody (AA 101-164)

Catalog No. ABIN672606

This anti-p21 antibody is a Rabbit Polyclonal antibody detecting p21 in WB, ELISA, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Human, Mouse, Rat and Sheep. This Primary Antibody has been cited in 5+ publications.

Quick Overview for p21 antibody (AA 101-164) (ABIN672606)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human, Mouse, Rat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-p21 Antibody

Binding Specificity: AA 101-164

Cross-Reactivity: Human, Mouse, Rat, Sheep

Predicted Reactivity: Dog,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CDKN1A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-p21 antibody (ABIN672606)

Jiang, Miao, Xiao, Zhang, Hu: "DAPT in the control of human hair follicle stem cell proliferation and differentiation." in: Post?py dermatologii i alergologii, Vol. 31, Issue 4, pp. 201-6, (2014) (PubMed).

Wu, Wang, Zong, Chen, Wang, Xu, Cui: "Simultaneous evaluation of p53 and p21 expression level for early cancer diagnosis using SERS technique." in: The Analyst, Vol. 138, Issue 12, pp. 3450-6, (2013) (PubMed).

Li, Dai, Ye, Wang, Xiao, Zheng, Chen, Luo, Liu: "Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition." in: Cancer cell international, Vol. 12, Issue 1, pp. 49, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A

Background:

Synonyms: P21, CDKI, CIP1, SDI1, Waf1, mda6, CAP2, Cdkn1, p21WAF, p21Cip1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, Cdkn1a

Background: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (By similarity).

Gene ID: 12575

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy