PCNA antibody (AA 185-261)

Catalog No. ABIN672756

This anti-PCNA antibody is a Rabbit Polyclonal antibody detecting PCNA in WB, ELISA, FACS, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Human, Mouse, Rat, Chicken, Rabbit and Sheep. This Primary Antibody has been cited in 10+ publications.

Quick Overview for PCNA antibody (AA 185-261) (ABIN672756)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PCNA Antibody

Binding Specificity: AA 185-261

Cross-Reactivity: Chicken, Human, Mouse, Rabbit, Rat, Sheep

Predicted Reactivity: Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCNA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PCNA antibody (ABIN672756)

Hu, Zuo, Li, Wang, Peng, Fang, Cui, Gao, Song, Zhou, Chen: "The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B 1." in: Scientific reports, Vol. 8, Issue 1, pp. 1770, (2018) (PubMed).

He, Bian, Ma, Wang, Wang, Zhao: "Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2." in: Oncology letters, Vol. 15, Issue 3, pp. 3977-3984, (2018) (PubMed).

Yin, Jiang, Peng, Cui, Zhou, He, Zuo, Ouyang, Fan, Fang: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." in: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, (2016) (PubMed).

Bi, Wang, Yang, Li, Liang, Han: "Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats." in: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 851-856, (2015) (PubMed).

Li, Zhao, Wang, Chen, Lin, Zhu, Zheng, Wu, Cheng, Li, Bai, Fan, Liu: "Urotensin II promotes atherosclerosis in cholesterol-fed rabbits." in: PLoS ONE, Vol. 9, Issue 4, pp. e95089, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).

Song, Yan, An, Hao, Guo, Zhang, Li, Li, Sun: "Potential contribution of SOCC to cerebral vasospasm after experimental subarachnoid hemorrhage in rats." in: Brain research, Vol. 1517, pp. 93-103, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy