Vimentin antibody (AA 371-466)

Catalog No. ABIN672786

This anti-Vimentin antibody is a Rabbit Polyclonal antibody detecting Vimentin in WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p) and IF (cc). Suitable for Human, Mouse, Rat, Pig, Cow and Monkey. This Primary Antibody has been cited in 51+ publications.

Quick Overview for Vimentin antibody (AA 371-466) (ABIN672786)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Vimentin Antibody

Binding Specificity: AA 371-466

Specificity: KO-Validated

Cross-Reactivity: Cow, Human, Monkey, Mouse, Pig, Rat

Predicted Reactivity: Cow,Chicken,Goat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Vimentin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Vimentin antibody (ABIN672786)

Pagani, Ghezzi, Ulisse, Rubio, Turrini, Garavaglia, Candiani, Castilletti, Ippolito, Poli, Broccoli, Panina-Bordignon, Vicenzi: "Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus." in: Scientific reports, Vol. 7, pp. 44286, (2018) (PubMed).

Smith, Zhang, Schiele, Khademhosseini, Kuo, Yelick: "Developing a biomimetic tooth bud model." in: Journal of tissue engineering and regenerative medicine, (2017) (PubMed).

Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, (2017) (PubMed).

Tanos, Saibene, Pipolo, Battaglia, Felisati, Rubio: "Isolation of putative stem cells present in human adult olfactory mucosa." in: PLoS ONE, Vol. 12, Issue 7, pp. e0181151, (2017) (PubMed).

Park, Sung, Jeong, Choi, Lee, Kwon, Jang, Park, Kim, Kim, Yoon, Ryoo, Kim: "Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation." in: Cell biochemistry and function, Vol. 35, Issue 7, pp. 392-400, (2017) (PubMed).

Wang, Cao, Liu, Du, Wang, Chen, Liu, Wu: "5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 46-56, (2017) (PubMed).

Djamali, Wilson, Sadowski, Zha, Niles, Hafez, Dorn, Mehner, Grimm, Hoffmann, Zhong, Fain, Reese: "Nox2 and Cyclosporine-Induced Renal Hypoxia." in: Transplantation, Vol. 100, Issue 6, pp. 1198-210, (2017) (PubMed).

Fagundes, Fernandes, Paraense, de Farias-Junior, Teixeira, Alves-Junior, Pinheiro, Crespo-López, Maia, Lima: "Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 7323627, (2017) (PubMed).

Yap, Digilio, McMahon, Roszkowska, Bott, Kruczek, Winckler: "Different Doublecortin (DCX) Patient Alleles Show Distinct Phenotypes in Cultured Neurons: EVIDENCE FOR DIVERGENT LOSS-OF-FUNCTION AND "OFF-PATHWAY" CELLULAR MECHANISMS." in: The Journal of biological chemistry, Vol. 291, Issue 52, pp. 26613-26626, (2017) (PubMed).

Khayat, Monteiro, Smith, Pagni, Zhang, Khademhosseini, Yelick: "GelMA-Encapsulated hDPSCs and HUVECs for Dental Pulp Regeneration." in: Journal of dental research, Vol. 96, Issue 2, pp. 192-199, (2017) (PubMed).

Wang, Nikhil, Viccaro, Chang, Jacobsen, Sandusky, Shah: "The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma." in: Journal of cell science, Vol. 130, Issue 6, pp. 1078-1093, (2017) (PubMed).

Wang, Jin, Fan, Wang, Xing, Niu: "Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene." in: PLoS ONE, Vol. 12, Issue 2, pp. e0172233, (2017) (PubMed).

Sucre, Wilkinson, Vijayaraj, Paul, Dunn, Alva-Ornelas, Gomperts: "A three-dimensional human model of the fibroblast activation that accompanies bronchopulmonary dysplasia identifies Notch-mediated pathophysiology." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 310, Issue 10, pp. L889-98, (2016) (PubMed).

Huang, Wen: "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." in: Molecular biology reports, Vol. 43, Issue 10, pp. 1069-78, (2016) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

Synonyms: HEL113, CTRCT3, Vimentin, VIM

Background: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis