Recombinant Notch1 antibody (Sortase,His tag,AVI tag)

Catalog No. ABIN6731040

This Mouse Monoclonal antibody specifically detects Notch1 in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant Notch1 antibody (Sortase,His tag,AVI tag) (ABIN6731040)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Notch1 antibody is conjugated to Sortase,His tag,AVI tag

Application: Western Blotting (WB)

Product Details anti-Notch1 Antibody (Sortase,His tag,AVI tag)

Purpose: AbFlex Notch1 antibody (rAb)

Brand: AbFlex®

Characteristics: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a recombinant protein corresponding to the intracellular domain of human Notch1.

Isotype: IgG2a

Application Details

Application Notes: WB: 1 - 2 μg/mL AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61147.

Comment:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Notch1

Background: AbFlex Notch1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Notch1 is a member of the Notch family of receptors, which make up a highly conserved cell signaling system. Notch1 functions as a ligand-activated transcription factor that directly transduces extracellular signals in the cell surface into changes in gene expression in the nucleus. Notch1 is membrane bound, serving as an extracellular receptor for the Delta and Serrate family of ligands. Upon activation, the intracellular domain is cleaved and rapidly translocates into the nucleus where it forms a transcriptional complex with the RBP-J DNA binding protein and activates gene expression via recruitment of co-activators of the MAML family. Notch receptors play a critical role in cell fate specification during development and participate in multiple biological processes. Notch signaling is required for undifferentiated embryonic stem cells (ESCs) to participate in the formation of all three embryonic germ layers.

Molecular Weight: 120 kDa

NCBI Accession: NP_060087

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development