Serotonin Receptor 3A antibody (N-Term)

Catalog No. ABIN6735761

The Rabbit Polyclonal anti-Serotonin Receptor 3A antibody (ABIN6735761) specifically detects Serotonin Receptor 3A in WB, IHC and IHC (p). The antibody is reactive with Human, Rat, Mouse, Dog and Cow samples.

Quick Overview for Serotonin Receptor 3A antibody (N-Term) (ABIN6735761)

Target: Serotonin Receptor 3A (HTR3A)

Reactivity: Human, Rat, Mouse, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Serotonin Receptor 3A Antibody

Binding Specificity: N-Term

Specificity: Human HTR3A / 5-HT3A

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HTR3A (P46098, NP_000860). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset (92%), Horse (91%), Opossum (90%), Hamster (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Serotonin Receptor 3A

Target: Serotonin Receptor 3A (HTR3A)

Alternative Name: HTR3A / 5-HT3A Receptor

Background: Name/Gene ID: HTR3A
Subfamily: Serotonin 5-HT3 receptor
Family: Ion channel

Synonyms: HTR3A, 5-HT3-A, 5-HT3R-A, 5-HT3 receptor, 5HT3 serotonin receptor, 5-HT3A, 5-HT3a receptor, 5-hydroxytryptamine receptor 3, 5-HT-3, 5-HT3, 5-HT3R, 5HT3R, HTR3, Serotonin receptor 3A

Gene ID: 3359

NCBI Accession: NP_000860

UniProt: P46098

Pathways: Synaptic Membrane