THRB antibody (AA 37-86)

Catalog No. ABIN6736434

This anti-THRB antibody is a Rabbit Polyclonal antibody detecting THRB in WB, IHC and IHC (p). Suitable for Human, Rat, Horse, Dog, Guinea Pig, Rabbit, Monkey and Pig.

Quick Overview for THRB antibody (AA 37-86) (ABIN6736434)

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Reactivity: Human, Rat, Horse, Dog, Guinea Pig, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-THRB Antibody

Binding Specificity: AA 37-86

Brand: IHC-plus™

Specificity: Human THRB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse (100%) Dog, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa37-86 of human THRB (P10828, NP_000452). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Horse (100%), Galago, Elephant, Dog, Guinea pig (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. This antibody was tested in a simple peptide-based ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for THRB

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Alternative Name: NR1A2 / THRB

Background: Name/Gene ID: THRB
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: THRB, C-erba beta2, C-ERBA-2, ERBA-BETA, GRTH, Oncogene ERBA2, THRB2, TR-BETA, THRB1, NR1A2, Thyroid hormone receptor beta, Trbeta1, Trbeta2, C-erba beta1, C-ERBA-BETA, ERBA2, PRTH, THR1, Thyroid hormone receptor, beta, Trbeta

Gene ID: 7068

NCBI Accession: NP_000452

UniProt: P10828

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound