PCK1 antibody (AA 330-379)

Catalog No. ABIN6736745

This anti-PCK1 antibody is a Rabbit Polyclonal antibody detecting PCK1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Rabbit, Horse, Monkey, Chicken and Hamster.

Quick Overview for PCK1 antibody (AA 330-379) (ABIN6736745)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Monkey, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PCK1 Antibody

Binding Specificity: AA 330-379

Brand: IHC-plus™

Specificity: Human PCK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine, Rabbit, Chicken (100%) Pig (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa330-379 of human PCK1 (P35558, NP_002582). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Opossum, Turkey, Zebra finch, Chicken (100%), Bat, Pig, Platypus, Stickleback (92%), Salmon (91%), Dog, Guinea pig, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background: Name/Gene ID: PCK1

Synonyms: PCK1, PEPCKC, Phosphopyruvate carboxylase, PEP carboxykinase, PEPCK-C, PEPCK1

Gene ID: 5105

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis