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RPS6 antibody (pSer235, Ser236) (Alexa Fluor 647)

This anti-RPS6 antibody is a Rabbit Monoclonal antibody detecting RPS6 in IF and FACS. Suitable for Human and Rat.
Catalog No. ABIN6737421

Quick Overview for RPS6 antibody (pSer235, Ser236) (Alexa Fluor 647) (ABIN6737421)

Target

See all RPS6 Antibodies
RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity

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Human, Rat

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This RPS6 antibody is conjugated to Alexa Fluor 647

Application

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Immunofluorescence (IF), Flow Cytometry (FACS)
  • Binding Specificity

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    pSer235, Ser236

    Specificity

    Detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. The antibody was conjugated to Alexa Fluor 647 under optimal conditions with an F/P ratio of 2-5. The Alexa Fluor 647 dye is maximally excited by red light (e. g. 633 nm He-Ne laser). Unconjugated antibody reacts with human, monkey, mouse and rat Phospho-S6 Ribosomal protein and it is expected that Phospho-S6 Ribosomal Protein (Ser235/236) Rabbit mAb (Alexa Fluor 647 conjugate) will also recognize Phospho-S6 in these species.

    Immunogen

    Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

    Type of Immunogen: Synthetic phospho-peptide

    Isotype

    IgG
  • Application Notes

    Approved: Flo (1:10), IF (1:10)

    Usage: Suitable for use in Immunofluorescence and Flow Cytometry. Flow Cytometry 1:10. Immunofluorescence: 1:10. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, less than 0.1 % sodium azide, 2 mg/mL BSA.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target

    RPS6 (Ribosomal Protein S6 (RPS6))

    Alternative Name

    RPS6 / S6

    Background

    Name/Gene ID: RPS6

    Synonyms: RPS6, 40S ribosomal protein S6, S6, Ribosomal protein S6, Phosphoprotein NP33

    Gene ID

    6194

    UniProt

    P62753

    Pathways

    Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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