RASSF1 antibody (AA 145-260) (Biotin)

Catalog No. ABIN673766

The Rabbit Polyclonal anti-RASSF1 antibody is suitable to detect RASSF1 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC (p) and IHC (fro).

Quick Overview for RASSF1 antibody (AA 145-260) (Biotin) (ABIN673766)

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASSF1 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RASSF1 Antibody (Biotin)

Binding Specificity: AA 145-260

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RASSF1

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RASSF1

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Alternative Name: RASSF1

Background:

Synonyms: 123F2, RDA32, NORE2A, RASSF1A, REH3P21, Ras association domain-containing protein 1, RASSF1

Background: Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF1 stimulation. Isoform A interacts with CDC2, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

Gene ID: 11186

UniProt: Q9NS23