MAP2K3 antibody (N-Term)

Catalog No. ABIN6738016

The Rabbit Polyclonal anti-MAP2K3 antibody has been validated for WB. It is suitable to detect MAP2K3 in samples from Human, Mouse, Rat, Monkey, Rabbit and Cow.

Quick Overview for MAP2K3 antibody (N-Term) (ABIN6738016)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP2K3 Antibody

Binding Specificity: N-Term

Specificity: Human MAP2K3 / MEK3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit (100%) Dog, Horse, Guinea pig (92%) Chicken (90%) Mouse, Rat, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human MAP2K3 (P46734-2, NP_002747). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Rabbit (100%), Galago, Elephant, Dog, Horse, Guinea pig (92%), Chicken (90%), Rat, Bovine, Opossum, Platypus (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Alternative Name: MAP2K3 / MEK3 / MKK3

Background: Name/Gene ID: MAP2K3
Subfamily: MAP2K
Family: Protein Kinase

Synonyms: MAP2K3, MAPKK 3, MAPKK3, PRKMK3, SAPK kinase 2, SAPKK-2, SAPKK2, SKK2, MAP kinase kinase 3, MEK 3, MEK3, MKK3, MAPK/ERK kinase 3

Gene ID: 5606

NCBI Accession: NP_002747

UniProt: P46734

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2