SPTLC1 antibody (AA 254-303)

Catalog No. ABIN6738265

The Rabbit Polyclonal anti-SPTLC1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SPTLC1 in samples from Human, Mouse, Rat, Rabbit, Guinea Pig, Monkey and Hamster.

Quick Overview for SPTLC1 antibody (AA 254-303) (ABIN6738265)

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTLC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SPTLC1 Antibody

Binding Specificity: AA 254-303

Specificity: Human SPTLC1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Guinea pig (100%) Dog, Xenopus, Zebrafish (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human SPTLC1 (O15269, NP_006406). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Guinea pig (100%), Dog, Bovine, Bat, Horse, Pig, Opossum, Lizard, Xenopus, Stickleback, Zebrafish (92%), Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPTLC1

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Alternative Name: SPTLC1 / HSN1

Background: Name/Gene ID: SPTLC1

Synonyms: SPTLC1, HSAN1, HLCB1, HSAN, LCB1, HSN1, LCB 1, Serine palmitoyltransferase 1, SPTI, SPT 1, Serine C-palmitoyltransferase, LBC1, SPT1

Gene ID: 10558

NCBI Accession: NP_006406

UniProt: O15269