INSIG1 antibody (AA 71-120)

Catalog No. ABIN6740654

The Rabbit Polyclonal anti-INSIG1 antibody (ABIN6740654) specifically detects INSIG1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Sheep, Pig, Hamster and Xenopus laevis samples.

Quick Overview for INSIG1 antibody (AA 71-120) (ABIN6740654)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Sheep, Pig, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-INSIG1 Antibody

Binding Specificity: AA 71-120

Specificity: Human INSIG1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Pig, Chicken (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human INSIG2 (Q9Y5U4, NP_057217). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Opossum, Xenopus (100%), Marmoset, Dog, Bat, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (93%), Seabass, Salmon, Smelt, Stickleback, Zebrafish (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: INSIG1

Background: Name/Gene ID: INSIG1

Synonyms: INSIG1, Cl-6, CL6, INSIG-1, Insulin induced gene 1, Insulin-induced gene 1 protein, INSIG-1 membrane protein

Gene ID: 3638

NCBI Accession: NP_057217

UniProt: O15503

Pathways: ER-Nucleus Signaling