INSIG1 antibody (AA 71-120)

Catalog No. ABIN6740654

This anti-INSIG1 antibody is a Rabbit Polyclonal antibody detecting INSIG1 in WB. Suitable for Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Sheep, Pig, Hamster and Xenopus laevis.

Quick Overview for INSIG1 antibody (AA 71-120) (ABIN6740654)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Human, Mouse, Rat, Cow, Goat, Horse, Rabbit, Sheep, Pig, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-INSIG1 Antibody

Binding Specificity: AA 71-120

Specificity: Human INSIG1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Pig, Chicken (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human INSIG2 (Q9Y5U4, NP_057217). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Opossum, Xenopus (100%), Marmoset, Dog, Bat, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (93%), Seabass, Salmon, Smelt, Stickleback, Zebrafish (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: INSIG1

Background: Name/Gene ID: INSIG1

Synonyms: INSIG1, Cl-6, CL6, INSIG-1, Insulin induced gene 1, Insulin-induced gene 1 protein, INSIG-1 membrane protein

Gene ID: 3638

NCBI Accession: NP_057217

UniProt: O15503

Pathways: ER-Nucleus Signaling