Crossover junction endonuclease EME1 (EME1) antibody

Catalog No. ABIN7862672

The Mouse Monoclonal anti-Crossover junction endonuclease EME1 antibody (Clone MTA31 7H2-1) (ABIN7862672) specifically detects Crossover junction endonuclease EME1 in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for Crossover junction endonuclease EME1 (EME1) antibody (ABIN7862672)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: MTA31 7H2-1

Product Details

Purpose: EME1 Antibody

Specificity: Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: His-tagged recombinant Eme1 of human origin

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1

Background:

Crossover junction endonuclease EME1, Essential Meiotic Endonuclease 1 Homolog 1/2, hMMS4, MMS4L, SLX2A

HEK293T or U-87 MG cells. Human tonsil.

Molecular Weight: 65kDa

Gene ID: 146956, 514330

UniProt: Q96AY2