NR0B1 antibody (AA 359-408)
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- Target See all NR0B1 Antibodies
- NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))
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Binding Specificity
- AA 359-408
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Reactivity
- Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Monkey, Zebrafish (Danio rerio), Chicken, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NR0B1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Human NR0B1 / DAX-1
- Cross-Reactivity
- Chicken, Dog (Canine), Mouse (Murine), Pig (Porcine), Rat (Rattus)
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Zebrafish (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa359-408 of human NR0B1 (Q9BG96, NP_000466). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard (100%), Galago, Hamster, Xenopus, Seabass, Salmon, Stickleback, Medaka (92%), Bat (91%), Zebrafish (85%), Catfish (81%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product NR0B1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- PBS, 0.09 % sodium azide, 2 % sucrose
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))
- Alternative Name
- NR0B1 / DAX1 (NR0B1 Products)
- Background
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Name/Gene ID: NR0B1
Subfamily: NR0 Knirps-like
Family: NHR
Synonyms: NR0B1, AHX, AHC, AHCH, DAX1, DSS, DAX 1, DAX-1, Adrenal hypoplasia congenita, Dosage-sensitive sex reversal, GTD, HHG, Nuclear hormone receptor, NROB1, Nuclear receptor DAX-1, SRXY2, Nuclear receptor DAX1, Dax-1 receptor - Gene ID
- 190
- NCBI Accession
- NP_000466
- UniProt
- P51843
- Pathways
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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