SIN3A antibody (N-Term)

Catalog No. ABIN6741541

This Rabbit Polyclonal antibody specifically detects SIN3A in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Dog, Pig, Bat, Hamster and Monkey.

Quick Overview for SIN3A antibody (N-Term) (ABIN6741541)

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Pig, Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIN3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIN3A Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human SIN3A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse (100%) Rabbit, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SIN3A (Q96ST3, NP_056292). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig (100%), Rabbit, Opossum, Guinea pig (92%), Zebra finch, Platypus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIN3A

Target: SIN3A (SIN3 homolog A, transcription regulator (SIN3A))

Alternative Name: SIN3A

Background: Name/Gene ID: SIN3A

Synonyms: SIN3A, DKFZP434K2235, KIAA0700

Gene ID: 25942

NCBI Accession: NP_056292

UniProt: Q96ST3

Pathways: Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha