NCOA3 antibody (AA 576-625)

Catalog No. ABIN6741542

The Rabbit Polyclonal anti-NCOA3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NCOA3 in samples from Human, Cow, Pig and Monkey.

Quick Overview for NCOA3 antibody (AA 576-625) (ABIN6741542)

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivity: Human, Cow, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NCOA3 Antibody

Binding Specificity: AA 576-625

Brand: IHC-plus™

Specificity: Human NCOA3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa576-625 of human NCOA3 (Q0VF45, NP_006525). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Pig (100%), Horse (92%), Marmoset, Bat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: NCOA3 / SRC-3 / AIB1

Background: Name/Gene ID: NCOA3

Synonyms: NCOA3, ACTR, AIB1, BHLHe42, CAGH16, CBP-interacting protein, CTG26, AIB-1, P/CIP, TNRC14, TRAM-1, Nuclear receptor coactivator 3, PCIP, RAC-3, SRC3, TNRC16, KAT13B, NCoA-3, SRC-3

Gene ID: 8202

NCBI Accession: NP_006525

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha