PKC gamma antibody (AA 539-588)

Catalog No. ABIN6741659

This Rabbit Polyclonal antibody specifically detects PKC gamma in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Rabbit, Monkey, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat and Pig.

Quick Overview for PKC gamma antibody (AA 539-588) (ABIN6741659)

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Monkey, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC gamma antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKC gamma Antibody

Binding Specificity: AA 539-588

Specificity: Human PRKCG / PKC-Gamma

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa539-588 of human PRKCG (P05129, NP_002730). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Armadillo (100%), Xenopus (92%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PKC gamma

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Alternative Name: PRKCG / PKC-Gamma

Background: Name/Gene ID: PRKCG
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCG, PKC-gamma, PRKC-gamma, Protein kinase C gamma, Protein kinase C gamma type, PKC Gamma, PKCC, PKCG, Pkcgamma, Protein kinase C, gamma, SCA14

Gene ID: 5582

NCBI Accession: NP_002730

UniProt: P05129

Pathways: WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGF Signaling