ERK1 antibody (AA 251-300)

Catalog No. ABIN6741660

The Rabbit Polyclonal anti-ERK1 antibody has been validated for WB. It is suitable to detect ERK1 in samples from Human, Mouse, Rat, Pig, Dog, Cow, Monkey, Zebrafish (Danio rerio), Horse and Guinea Pig.

Quick Overview for ERK1 antibody (AA 251-300) (ABIN6741660)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow, Monkey, Zebrafish (Danio rerio), Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERK1 Antibody

Binding Specificity: AA 251-300

Specificity: Human MAPK3 / ERK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse (100%) Rabbit (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human MAPK3 (P27361, NP_002737). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%), Elephant, Bat, Rabbit, Opossum (92%), Stickleback (91%), Platypus, Salmon, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 / ERK1

Background: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gene ID: 5595

NCBI Accession: NP_002737

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins