p53 antibody (AA 36-85)
Quick Overview for p53 antibody (AA 36-85) (ABIN6742186)
Target
See all p53 (TP53) AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Binding Specificity
- AA 36-85
-
Specificity
- Human TP53 / p53
-
Predicted Reactivity
- Percent identity by BLAST analysis: Human (100%).
-
Purification
- Immunoaffinity purified
-
Immunogen
-
Synthetic peptide located between aa36-85 of human TP53 (P04637, NP_000537). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%).
Type of Immunogen: Synthetic peptide
-
-
-
-
Application Notes
-
Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Not recommended for: IHC-P -
Comment
-
Target Species of Antibody: Human
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Distilled water
-
Concentration
- Lot specific
-
Buffer
- Lyophilized from PBS with 2 % sucrose
-
Handling Advice
- Avoid repeat freeze-thaw cycles.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
-
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
-
-
- p53 (TP53) (Tumor Protein P53 (TP53))
-
Alternative Name
- TP53 / p53
-
Background
-
Name/Gene ID: TP53
Family: P53
Synonyms: TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1 -
Gene ID
- 7157
-
NCBI Accession
- NP_000537
-
UniProt
- P04637
-
Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target
-