AKT2 antibody (C-Term)

Catalog No. ABIN6742187

The Rabbit Polyclonal anti-AKT2 antibody has been validated for WB. It is suitable to detect AKT2 in samples from Human, Mouse, Rat, Dog, Chicken, Monkey, Cow, Pig, Horse, Guinea Pig, Xenopus laevis, Hamster, Sheep, Rabbit, Zebrafish (Danio rerio), Bat and Goat.

Quick Overview for AKT2 antibody (C-Term) (ABIN6742187)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Monkey, Cow, Pig, Horse, Guinea Pig, Xenopus laevis, Hamster, Sheep, Rabbit, Zebrafish (Danio rerio), Bat, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKT2 Antibody

Binding Specificity: C-Term

Specificity: Human AKT2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human AKT2 (P31751, NP_001617). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Dog, Bovine, Bat, Goat, Hamster, Elephant, Panda, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Salmon, Zebrafish, Stickleback (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2

Background: Name/Gene ID: AKT2
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT2, PKB beta, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, Rac protein kinase beta, RAC-BETA, HIHGHH, PKBB

Gene ID: 208

NCBI Accession: NP_001617

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling