CHEK1 antibody (AA 251-300)

Catalog No. ABIN6742296

This Rabbit Polyclonal antibody specifically detects CHEK1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Chicken, Rabbit, Horse, Monkey, Pig, Guinea Pig, Bat and Hamster.

Quick Overview for CHEK1 antibody (AA 251-300) (ABIN6742296)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Rabbit, Horse, Monkey, Pig, Guinea Pig, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 251-300

Specificity: Human CHEK1 / CHK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Horse, Pig, Guinea pig (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human CHEK1 (O14757, NP_001265). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Guinea pig, Chicken (100%), Bovine, Platypus (92%), Zebra finch, Xenopus (91%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1 / CHK1

Background: Name/Gene ID: CHEK1
Subfamily: NIM1
Family: Protein Kinase

Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1

Gene ID: 1111

NCBI Accession: NP_001265

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair