PPARA antibody (AA 51-100)

Catalog No. ABIN6742339

The Rabbit Polyclonal anti-PPARA antibody is suitable to detect PPARA in samples from Human, Horse, Cow, Goat, Guinea Pig, Pig, Monkey and Chicken. It has been validated for WB.

Quick Overview for PPARA antibody (AA 51-100) (ABIN6742339)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivity: Human, Horse, Cow, Goat, Guinea Pig, Pig, Monkey, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPARA Antibody

Binding Specificity: AA 51-100

Specificity: Human PPARA / PPAR

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Goat, Horse, Pig, Guinea pig, Chicken (100%) Mouse, Rat, Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa51-100 of human PPARA (Q07869, NP_005027). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Goat, Elephant, Panda, Bovine, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken (100%), Mouse, Rat, Hamster, Dog, Bat, Opossum (92%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPAR alpha

Background: Name/Gene ID: PPARA
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARA, PPAR Alpha, PPARalpha, PPAR-alpha, NR1C1, HPPAR, PPAR

Gene ID: 5465

NCBI Accession: NP_005027

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C