PIAS1 antibody (N-Term)

Catalog No. ABIN6742834

The Rabbit Polyclonal anti-PIAS1 antibody (ABIN6742834) specifically detects PIAS1 in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Cow, Dog, Rabbit, Bat, Guinea Pig, Monkey, Zebrafish (Danio rerio), Pig, Chicken and Xenopus laevis samples.

Quick Overview for PIAS1 antibody (N-Term) (ABIN6742834)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Rabbit, Bat, Guinea Pig, Monkey, Zebrafish (Danio rerio), Pig, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIAS1 Antibody

Binding Specificity: N-Term

Specificity: Human PIAS1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PIAS1 (O75925, NP_057250). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Xenopus, Zebrafish (100%), Sea squirt (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: PIAS1

Background: Name/Gene ID: PIAS1

Synonyms: PIAS1, AR interacting protein, DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, GU/RH-II, Gu-binding protein, GBP, ZMIZ3

Gene ID: 8554

NCBI Accession: NP_057250

UniProt: O75925

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C