HLA-E antibody (AA 251-358)

Catalog No. ABIN674334

This anti-HLA-E antibody is a Rabbit Polyclonal antibody detecting HLA-E in WB, FACS, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for HLA-E antibody (AA 251-358) (ABIN674334)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-E antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HLA-E Antibody

Binding Specificity: AA 251-358

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HLA E

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HLA-E antibody (ABIN674334)

Sun, Xu, Huang, Huang, Wen, Zhang, Wang, Song, Zheng, Sun, Wei, Xiao, Sun, Tian: "High NKG2A expression contributes to NK cell exhaustion and predicts a poor prognosis of patients with liver cancer." in: Oncoimmunology, Vol. 6, Issue 1, pp. e1264562, (2017) (PubMed).

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Alternative Name: HLA E

Background:

Synonyms: MHC, QA1, EA1.2, EA2.1, HLA-6.2, HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E, HLA-E, HLAE

Background: HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Gene ID: 3133

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response