TRPM2 antibody (AA 431-480)

Catalog No. ABIN490062

Product Details anti-TRPM2 Antibody

Target: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Binding Specificity: AA 431-480

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TRPM2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Horse (92%) Guinea pig (87%) Chicken (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa431-480 of human TRPM2 (O94759, NP_003298). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat (100%), Galago, Marmoset, Elephant, Bovine, Bat, Horse (92%), Guinea pig (87%), Panda, Platypus, Lizard (85%), Turkey, Zebra finch, Chicken (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 - 20 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRPM2

Target: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Alternative Name: TRPM2 (TRPM2 Products)

Background: Name/Gene ID: TRPM2
Subfamily: Calcium channel - TRP
Family: Ion Channel

Synonyms: TRPM2, EREG1, LTrpC-2, LTRPC2, NUDT9L1, KNP3, NUDT9H, Trrp7

Gene ID: 7226

NCBI Accession: NP_003298

UniProt: O94759