MGMT antibody (AA 143-192)

Catalog No. ABIN6744712

This anti-MGMT antibody is a Rabbit Polyclonal antibody detecting MGMT in WB. Suitable for Human, Rat, Mouse, Monkey, Dog, Guinea Pig, Horse, Bat, Hamster and Pig.

Quick Overview for MGMT antibody (AA 143-192) (ABIN6744712)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human, Rat, Mouse, Monkey, Dog, Guinea Pig, Horse, Bat, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MGMT Antibody

Binding Specificity: AA 143-192

Specificity: Human MGMT

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human MGMT (P16455, NP_002403). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Horse, Guinea pig (100%), Elephant (92%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: Name/Gene ID: MGMT

Synonyms: MGMT

Gene ID: 4255

NCBI Accession: NP_002403

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus