MGMT antibody (AA 143-192)

Catalog No. ABIN6744712

The Rabbit Polyclonal anti-MGMT antibody (ABIN6744712) specifically detects MGMT in WB. The antibody is reactive with Human, Rat, Mouse, Monkey, Dog, Guinea Pig, Horse, Bat, Hamster and Pig samples.

Quick Overview for MGMT antibody (AA 143-192) (ABIN6744712)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human, Rat, Mouse, Monkey, Dog, Guinea Pig, Horse, Bat, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MGMT Antibody

Binding Specificity: AA 143-192

Specificity: Human MGMT

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human MGMT (P16455, NP_002403). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bat, Horse, Guinea pig (100%), Elephant (92%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: Name/Gene ID: MGMT

Synonyms: MGMT

Gene ID: 4255

NCBI Accession: NP_002403

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus