GRK2 antibody (AA 35-84)

Catalog No. ABIN6744925

The Rabbit Polyclonal anti-GRK2 antibody has been validated for WB. It is suitable to detect GRK2 in samples from Human, Mouse, Rat, Cow, Dog, Monkey, Pig, Guinea Pig, Horse, Bat and Hamster.

Quick Overview for GRK2 antibody (AA 35-84) (ABIN6744925)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey, Pig, Guinea Pig, Horse, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRK2 Antibody

Binding Specificity: AA 35-84

Specificity: Human ADRBK1 / GRK2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human ADRBK1 (P25098, NP_001610). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Lizard (100%), Elephant, Turkey, Zebra finch, Chicken (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK2

Background: Name/Gene ID: ADRBK1
Subfamily: GPRK
Family: Protein Kinase

Synonyms: ADRBK1, BARK1, Beta-ARK-1, BETA-ARK1, BARK, Betaark1, GRK2

Gene ID: 156

NCBI Accession: NP_001610

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling