MED25 antibody (AA 33-82)

Catalog No. ABIN6745504

This Rabbit Polyclonal antibody specifically detects MED25 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig, Zebrafish (Danio rerio), Monkey and Xenopus laevis.

Quick Overview for MED25 antibody (AA 33-82) (ABIN6745504)

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig, Zebrafish (Danio rerio), Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED25 Antibody

Binding Specificity: AA 33-82

Specificity: Human MED25

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Xenopus, Zebrafish (100%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa33-82 of human MED25 (Q71SY5, NP_112235). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Pig, Xenopus, Stickleback, Zebrafish (100%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED25

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Alternative Name: MED25

Background: Name/Gene ID: MED25

Synonyms: MED25, ACID1, CMT2B2, TCBAP0758, Mediator complex subunit 25, PTOV2, ARC92, p78

Gene ID: 81857

NCBI Accession: NP_112235

UniProt: Q71SY5

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response