Bcl-2 antibody (AA 85-134)

Catalog No. ABIN6745511

The Rabbit Polyclonal anti-Bcl-2 antibody has been validated for WB. It is suitable to detect Bcl-2 in samples from Human, Mouse, Rat, Pig, Monkey, Rabbit, Sheep, Dog, Cow, Horse and Hamster.

Quick Overview for Bcl-2 antibody (AA 85-134) (ABIN6745511)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat, Pig, Monkey, Rabbit, Sheep, Dog, Cow, Horse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 85-134

Specificity: Human BCL2 / Bcl-2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Pig (100%) Guinea pig (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa85-134 of human BCL2 (P10415, NP_000624). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum (100%), Guinea pig (92%), Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL2 / Bcl-2

Background: Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50

Gene ID: 596

NCBI Accession: NP_000624

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling