p53 antibody (N-Term)
Quick Overview for p53 antibody (N-Term) (ABIN6745699)
Target
See all p53 (TP53) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Brand
- IHC-plus™
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Specificity
- Human TP53 / p53
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Predicted Reactivity
- Percent identity by BLAST analysis: Human, Bovine (100%) Horse (90%).
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic peptide from N-Terminus of human TP53 (P04637, NP_000537). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Sheep, Bovine, Pig, Zebu (100%), Monkey, Galago, Marmoset, Cat, Horse, Guinea pig (90%).
Type of Immunogen: Synthetic peptide
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Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Western Blot: using 293T cell lysates, suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Alternative Name
- TP53 / p53
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Background
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Name/Gene ID: TP53
Family: P53
Synonyms: TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1 -
Gene ID
- 7157
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NCBI Accession
- NP_000537
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UniProt
- P04637
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Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target
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