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p53 antibody (N-Term)

This anti-p53 antibody is a Rabbit Polyclonal antibody detecting p53 in WB, IHC and IHC (p). Suitable for Human, Cow, Pig and Sheep.
Catalog No. ABIN6745699

Quick Overview for p53 antibody (N-Term) (ABIN6745699)

Target

See all p53 (TP53) Antibodies
p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity

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Human, Cow, Pig, Sheep

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This p53 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    N-Term

    Brand

    IHC-plus™

    Specificity

    Human TP53 / p53

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Bovine (100%) Horse (90%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from N-Terminus of human TP53 (P04637, NP_000537). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Sheep, Bovine, Pig, Zebu (100%), Monkey, Galago, Marmoset, Cat, Horse, Guinea pig (90%).

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Western Blot: using 293T cell lysates, suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.
  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Alternative Name

    TP53 / p53

    Background

    Name/Gene ID: TP53
    Family: P53

    Synonyms: TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1

    Gene ID

    7157

    NCBI Accession

    NP_000537

    UniProt

    P04637

    Pathways

    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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