Leptin antibody (N-Term)

Catalog No. ABIN6745724

This Rabbit Polyclonal antibody specifically detects Leptin in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Cow, Horse, Dog, Rabbit and Hamster.

Quick Overview for Leptin antibody (N-Term) (ABIN6745724)

Target: Leptin (LEP)

Reactivity: Human, Mouse, Cow, Horse, Dog, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Leptin Antibody

Binding Specificity: N-Term

Specificity: Human LEP / Leptin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Rat (92%) Sheep, Goat (90%) Dog, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LEP (P41159, NP_000221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Hamster (100%), Galago, Marmoset, Rat, Opossum (92%), Sheep, Goat (90%), Monkey, Elephant, Zebu, Dog, Cat, Bovine (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB

Gene ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process