Leptin antibody (C-Term)

Catalog No. ABIN604739

Product Details anti-Leptin Antibody

Target: Leptin (LEP)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Goat, Sheep, Chicken, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human LEP / Leptin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Bovine, Goat, Horse, Chicken (100%) Rabbit, Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human LEP (P41159, NP_000221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Hamster, Elephant, Zebu, Bovine, Horse, Turkey, Chicken (100%), Orangutan, Cat, Rabbit, Pig (92%), Panda (85%), Marmoset (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin (LEP Products)

Synonyms: ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody

Background: Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB

Gene ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process