CD22 antibody (AA 45-94)

Catalog No. ABIN6746695

The Rabbit Polyclonal anti-CD22 antibody has been validated for WB. It is suitable to detect CD22 in samples from Human. There are 7+ publications available.

Quick Overview for CD22 antibody (AA 45-94) (ABIN6746695)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD22 Antibody

Binding Specificity: AA 45-94

Specificity: Human CD22

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa45-94 of human CD22 (P20273, NP_001762). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Gibbon, Monkey (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-CD22 antibody (ABIN6746695)

Waite, Floyd, Arbour-Reily, Stephens: "Interferon-gamma-induced regulation of peroxisome proliferator-activated receptor gamma and STATs in adipocytes." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7062-8, (2001) (PubMed).

Bartoli, Gu, Tsai, Venema, Brooks, Marrero, Caldwell: "Vascular endothelial growth factor activates STAT proteins in aortic endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33189-92, (2000) (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

Dent, Hu-Li, Paul, Staudt: "T helper type 2 inflammatory disease in the absence of interleukin 4 and transcription factor STAT6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13823-8, (1998) (PubMed).

Heim: "The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus." in: European journal of clinical investigation, Vol. 26, Issue 1, pp. 1-12, (1996) (PubMed).

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, (1996) (PubMed).

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, (1994) (PubMed).

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: Name/Gene ID: CD22
Family: Immunoglobulin

Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM

Gene ID: 933

NCBI Accession: NP_001762

UniProt: P20273