FOXO1 antibody (AA 373-422)

Catalog No. ABIN768735

Product Details anti-FOXO1 Antibody

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Binding Specificity: AA 373-422

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FOXO1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig (100%) Dog, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa373-422 of human FOXO1 (Q12778, NP_002006). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Rabbit, Horse, Pig, Opossum (100%), Panda, Dog, Bat, Guinea pig, Platypus (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1 / FKHR (FOXO1 Products)

Background: Name/Gene ID: FOXO1
Family: Transcription factor

Synonyms: FOXO1, FKHR, Forkhead in rhabdomyosarcoma, FOXO1A, FKH1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead box O1, Forkhead box o1a, Forkhead domain protein fkhr

Gene ID: 2308

NCBI Accession: NP_002006

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling