PLK1 antibody (C-Term)

Catalog No. ABIN6747101

This anti-PLK1 antibody is a Rabbit Polyclonal antibody detecting PLK1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rabbit, Guinea Pig and Hamster.

Quick Overview for PLK1 antibody (C-Term) (ABIN6747101)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse, Rabbit, Guinea Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLK1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human PLK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Rat, Dog, Bovine, Horse (92%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human PLK1 (P53350, NP_005021). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Mouse, Hamster, Elephant, Rabbit (100%), Galago, Marmoset, Rat, Dog, Bovine, Bat, Horse, Opossum (92%), Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1 / PLK-1

Background: Name/Gene ID: PLK1
Subfamily: Polo
Family: Protein Kinase

Synonyms: PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1

Gene ID: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cell Division Cycle, M Phase