FBXW7 antibody (C-Term)

Catalog No. ABIN6747109

The Rabbit Polyclonal anti-FBXW7 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FBXW7 in samples from Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Drosophila melanogaster, Monkey, Xenopus laevis, Hamster, Bat and Chicken.

Quick Overview for FBXW7 antibody (C-Term) (ABIN6747109)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Drosophila melanogaster, Monkey, Xenopus laevis, Hamster, Bat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FBXW7 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human Fbxw7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human FBXW7 (Q969H0-4, NP_001013433). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish, Drosophila, Beetle (100%), Stickleback, Nematode (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7 / FBW7

Background: Name/Gene ID: FBXW7
Family: F-Box

Synonyms: FBXW7, AGO, Archipelago homolog, Archipelago, CDC4, F-box protein FBW7, FBXW6, F-box protein FBX30, F-box protein SEL-10, HCdc4, Homolog of C elegans sel-10, FBW6, FBX30, FBW7, HAgo, SEL-10, SEL10

Gene ID: 55294

NCBI Accession: NP_001013433

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway