Aurora A antibody (C-Term)

Catalog No. ABIN6748546

This Rabbit Polyclonal antibody specifically detects Aurora A in WB, IHC and IHC (p). It exhibits reactivity toward Human, Cow and Sheep.

Quick Overview for Aurora A antibody (C-Term) (ABIN6748546)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aurora A Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human AURKA / Aurora-A

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Sheep, Bovine (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human AURKA (O14965, NP_003591). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey, Marmoset (86%), Sheep, Bovine (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA / Aurora-A

Background: Name/Gene ID: AURKA
Subfamily: Aurora/IPL1-like
Family: Protein Kinase

Synonyms: AURKA, AIK, AIRK1, ARK1, Aurora-A kinase, Aurora kinase A, Aurora/IPL1-like kinase, AYK1, AURA, AURORA2, Breast tumor-amplified kinase, Breast-tumor-amplified kinase, BTAK, HARK1, IPL1-related kinase, Serine/threonine kinase 6, STK7, STK6, PPP1R47, ARK-1, Aurora 2, Aurora-A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, IAK1, Serine/threonine kinase 15, STK15

Gene ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization