H2AFX antibody (C-Term)

Catalog No. ABIN6748635

This anti-H2AFX antibody is a Rabbit Polyclonal antibody detecting H2AFX in WB, IHC and IHC (p). Suitable for Human, Rat, Rabbit, Monkey and Bat.

Quick Overview for H2AFX antibody (C-Term) (ABIN6748635)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Rat, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-H2AFX Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human H2AFX / H2AX

Predicted Reactivity: Percent identity by BLAST analysis: Bovine (92%) Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human H2AFX (P16104, NP_002096). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Bat, Rabbit (100%), Dog, Bovine, Guinea pig (92%), Galago, Rat, Hamster (85%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AX

Background: Name/Gene ID: H2AFX

Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone

Gene ID: 3014

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus