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Manic Fringe antibody (AA 211-260)

This anti-Manic Fringe antibody is a Rabbit Polyclonal antibody detecting Manic Fringe in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Horse, Cow, Rabbit, Bat and Monkey.
Catalog No. ABIN1913322

Quick Overview for Manic Fringe antibody (AA 211-260) (ABIN1913322)

Target

See all Manic Fringe (MFNG) Antibodies
Manic Fringe (MFNG) (MFNG)

Reactivity

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Human, Mouse, Rat, Dog, Horse, Cow, Rabbit, Bat, Monkey

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Manic Fringe antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    AA 211-260

    Brand

    IHC-plus™

    Specificity

    Human MFNG

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa211-260 of human MFNG (O00587, NP_002396). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bat, Rabbit, Horse (100%), Galago, Guinea pig, Platypus (92%), Dog, Opossum (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    Manic Fringe (MFNG) (MFNG)

    Alternative Name

    MFNG / Manic Fringe

    Background

    Name/Gene ID: MFNG

    Synonyms: MFNG, Manic fringe

    Gene ID

    4242

    NCBI Accession

    NP_002396

    Pathways

    Notch Signaling
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